Liquid chromatography duba bawul Ruby yumbu ruwa maye gurbin

Yaushe za a maye gurbin bawul ɗin duba?
① "Lost Prime" yana bayyana lokacin da tsarin ke gudana yana nuna matsin tsarin yayi ƙasa da ƙasa, da ƙasa da matsa lamba na baya da ake buƙata don aikin chromatography na ruwa na yau da kullun.Yawanci yana faruwa ne ta hanyar gurɓatar bawul ɗin duba a cikin famfo, ko ƙananan kumfa sun kasance a cikin bawul ɗin da ke kaiwa ga jiko maras kyau.A wannan lokacin, abin da ya kamata mu yi shi ne yin ƙoƙari don kawar da ƙananan kumfa ta hanyar aiki na minti biyar na "Wet Prime".Idan wannan bayani ya kasa, ya kamata mu cire rajistan bawul, kuma tsaftace shi ultrasonically da ruwa sama da 80 ℃.Ana ba da shawarar maye gurbin cak ɗin bawul ɗin duba idan maimaita tsaftacewa ba ta da tasiri.

② Ya zama cewa akwai kumfa a cikin famfo ko duba bawul lokacin da matsa lamba na tsarin ke canzawa sosai.Za mu iya yin aiki da "Wet Prime" na minti 5-10, don wanke kumfa tare da babban adadin ruwa.Idan sama hanya ba ya aiki, ya kamata mu cire rajistan bawul, da kuma tsabtace shi ultrasonically da ruwa sama da 80 ℃.Ana ba da shawarar maye gurbin cak ɗin bawul ɗin duba idan maimaita tsaftacewa ba ta da tasiri.

③ Lokacin da aka sami matsala tare da sake haifar da allurar tsarin, da farko kiyaye lokacin riƙewa.Idan akwai matsala tare da lokacin riƙewa, duba jujjuyawar matsa lamba tsarin al'ada ne ko a'a.A al'ada, a cikin adadin 1ml / min, tsarin tsarin kayan aiki ya kamata ya zama 2000 ~ 3000psi.(Akwai bambance-bambancen rabo dangane da nau'ikan ginshiƙan chromatographic da matakan wayar hannu.) Yana da al'ada cewa saurin matsa lamba yana cikin 50psi.Daidaitaccen madaidaicin tsarin matsi mai kyau yana cikin 10psi.A ƙarƙashin yanayin cewa canjin matsa lamba ya yi girma, muna buƙatar yin la'akari da yiwuwar cewa bawul ɗin duba ya gurɓata ko yana da kumfa, sannan mu magance shi.

Yaushe za a yi amfani da bawul ɗin duba yumbu?
Akwai batun daidaitawa tsakanin ruby ​​check valve na 2690/2695 da wasu nau'ikan acetonitrile.Musamman halin da ake ciki shine: lokacin amfani da 100% acetonitrile, barin shi cikin dare, kuma ci gaba da fara gwaje-gwaje a rana mai zuwa, babu wani ruwa da ke fitowa daga famfo.Wannan saboda an manne jikin jikin ruby ​​check valve da ƙwallon ruby ​​bayan an jiƙa cikin tsantsar acetonitrile.Ya kamata mu cire bawul ɗin rajistan kuma mu buga shi da sauƙi ko bi da ultrasonically.Lokacin girgiza bawul ɗin dubawa da jin ƙaramar sauti, wannan yana nufin bawul ɗin rajistan ya dawo daidai.Yanzu mayar da bawul ɗin duba.Ana iya yin gwaje-gwaje akai-akai bayan mintuna 5 "Wet Prime".

Don guje wa wannan matsala a bin gwaje-gwaje, ana ba da shawarar yin amfani da bawul ɗin duba yumbu.