Rukunin Fatalwa-Sniper Chromasir HPLC UPLC shafi yana kawar da kololuwar fatalwa
Fatalwa-sniper shafi an kera shi musamman don kawar da kololuwar fatalwa. Kololuwar fatalwa asalinsu ba a san su ba a cikin na'urar chromatogram, yawanci ana lura da su yayin aiwatar da rabuwar chromatographic a cikin gradient elution ko aiki na dogon lokaci. Faruwar kololuwar fatalwa yana da tasiri mai zurfi akan gwaje-gwajen manazarta. Fatalwa-sniper shafi yana faruwa don magance waɗannan matsalolin kuma sanya tsarin gwaji ya fi dacewa da inganci. Ana iya amfani da wannan ginshiƙi a cikin matsanancin yanayi kuma yana nuna tasiri mai girma. Tabbas hanya ce mai kyau don kawar da tsangwama daga kololuwar fatalwa akan tabbatar da hanya da kuma binciken abubuwan gano abubuwa.
Bangaren No. | Girma | Ƙarar | Aikace-aikace |
Saukewa: MC5046091P | 50×4.6mm | kusan 800ul | HPLC |
Saukewa: MC3546092P | 35×4.6mm | ku 580ul | HPLC |
Saukewa: MC5021093P | 50×2.1mm | kusan 170ul | Farashin UPLC |
Saukewa: MC3040096P | 30×4.0mm | ku 380ul | Ƙananan ginshiƙi na HPLC |
Shigarwa
Aikace-aikace da Sakamako
1. Idan an shirya nazarin batch a cikin tsarin HPLC, ana bada shawara don ƙara ƙarin lokacin ma'auni na kimanin 5 min - 10 min a cikin yanayin chromatographic ku, don tasirin ƙarar ginshiƙan fatalwa-sniper.
2. Don sababbin ginshiƙai, zubar da su tare da 100% acetonitrile a yawan adadin 0.5ml / min na 4 hours kafin amfani.
3. Ion-biyu reagents a cikin mobile lokaci, za a iya tunawa da fatalwa-sniper ginshiƙi, wanda zai iya rinjayar da riƙe lokacin da kololuwa siffar your manufa. Da fatan za a yi amfani da hankali a ƙarƙashin irin wannan lokacin wayar hannu.
4. Rayuwar ginshiƙi ya dogara da yanayin nazari, kamar lokacin wayar hannu. da sauran ƙarfi tsarki, da kuma gurbace na kayan aiki. Da fatan za a maye gurbin ginshiƙin maharbi akai-akai don tabbatar da aiki.
5. Don maye gurbin ginshiƙin maharbi ana ba da shawarar idan ɗaukar tasirin ya yi muni ko buƙatun ba za a iya gamsar da su ba.
6. A matsayin wani ɓangare na tsarkakewa zuwa ruwa chromatograph, fatalwa-sniper shafi na iya tace m barbashi da kuma kawar da kwayoyin gurbatawa kafin injector. Rukunin maharbi na fatalwa kuma yana ba da kayan kida da ginshiƙin chromatographic tare da mafi kyawun kariya, kuma yana kammala chromatogram.
7. Idan lokaci na wayar hannu ya ƙunshi gishiri mai buffer, kafin da bayan amfani, watsa don yin ruwa tare da 10% maganin lokaci na kwayoyin halitta (10% methanol ko acetonitrile), don kauce wa gishiri mai gishiri da kuma toshe ginshiƙi.
8. Da fatan za a lura cewa ba duk kololuwar fatalwa ba ne za a iya kama su ta shafi Ghost-Sniper.
9. Idan ginshiƙi ya bar ba a amfani da shi na dogon lokaci, ana bada shawara don ajiye shi a cikin maganin ruwa mai ruwa (70% methanol ko acetonitrile). Kuma da fatan za a zubar da shi da 100% acetonitrile a cikin adadin 0.5 ml/min na awa 1 kafin amfani.